Coding

Part:BBa_K3786003:Design

Designed by: Nathalia Raquel de Souza Fernandes   Group: iGEM21_Paris_Bettencourt   (2021-10-21)


Min operon (min C, D, E)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 199
    Illegal PstI site found at 1146
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 199
    Illegal PstI site found at 1146
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 199
    Illegal PstI site found at 1146
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 199
    Illegal PstI site found at 1146
    Illegal NgoMIV site found at 821
    Illegal AgeI site found at 404
    Illegal AgeI site found at 700
    Illegal AgeI site found at 1587
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 9
    Illegal SapI site found at 567


Design Notes

Design was made for min mutant strains


Source

Min operon is amplified from E.coli genome.

References